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Peter Hoyt
| Research Area | Selected Publications | Lab Members |
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Title | Director, Array and Bioinformatics Core Facility |
| Office | 350A NRC | |
| Phone | 405-744- 6206 | |
| peter.r.hoyt@okstate.edu | ||
| Degree | Ph.D. Human Genetics/Cell Biology. 1988. University of Texas Medical Branch. | |
| Website | http://microarray.biochem.okstate.edu http://bioinfo.biochem.okstate.edu/ |
Assessment of epigenetic and metabolic affectors in whole-cell biology using advanced "-omic" technologies
Research includes identifying metabolic effects of low dose (<10cGY) ionizing radiation in skin of different inbred mouse strains. Strains show remarkable variability in genome-wide transcription affecting humoral immunity, cell cycling, apoptosis and the intracellular signaling cascade. Using microarrays and bionanotechnology, we are examining the response of cells impaled onto vector-derivitized carbon nanofibers. Surviving impaled cells are spatially fixed and express proteins via this transfection method. A novel mechanism of celluar damage assessment associated with membrane healing is being investigated. Other interests include identifying control of cell determination events during differentiation of pluripotent (hematopoietic) and omnipotent (embryonic) mouse stem cells. Genes and miRNA involved in determination will be studied simultaneously on microarrays. Finally, novel informatics and array technologies are being developed for high-throughput cellular analyses.
Selected Publications (top)
Mann D. G., McKnight T. E., McPherson J. T., Hoyt P. R., Melechko A. V., Simpson M. L., Sayler G. S. 2008. Inducible RNA Interference-Mediated Gene Silencing Using Nanostructured Gene Delivery Arrays. ACS Nano 2: 69-76.
Hoyt, P.R. and Conners, R. Optimizing hygroscopic printing buffers for large microarray probe libraries by on-site humidity equilibration. Manuscript in Preparation.
Hoyt, P. R., Melechenko, A. V. and McKnight, T. E. Cell systems responses of CHO-cells impaled on vertically arrayed nanofibers. Manuscript in preparation.
Hoyt et al. Genetic Background Effects on Transcription during Low Dose Radiation Response in Mouse Skin. Manuscript in preparation.
Hoyt, P. R., Doktycz, M. J., Tack, L. (2006) Preparation of cDNA probes for DNA microarrays. In: DNA Sequencing II: Isolation and Preparation. J. Kieleczawa ed. Jones and Bartlett Publishers.
Hoyt, P. R., Doktycz, M. J., (2004) Optimized Beadmilling of Tissues for High-Throughput RNA Production and Microarray-based Analyses. Analytical Biochemistry 332: 100-108.
Hoyt, P. R., Doktycz, M. J., K. L. Beattie, and M. S. Greeley, Jr. (2003) DNA microarrays detect 4-nonylphenol-induced alterations in gene expression during zebrafish early development. Ecotoxicology. 12: 469-474.
Doktycz, M. J., Sullivan, C. J., Hoyt, P. R., Pelletier, D. A., Wu, S., and Allison, D. P. (2003) AFM imaging of bacteria in liquid media immobilized on gelatin coated mica surfaces. Ultramicroscopy. 97:209-216.
Hoyt, P. R., Tack, L., Jones, B. H., Van Dinther, J., Staat, S., and Doktycz, M. J. (2003) Automated high-throughput probe production for DNA microarray analysis. BioTechniques. 34(2): 402-407.
Hoyt, P. R., Doktycz, M. J., Warmack, R. J., and Allison, D. P. (2001) Spin column isolation of protein-DNA interactions from complex protein mixtures for AFM imaging. Ultramicroscopy. 86:139-143.
Hoyt, P. R., Doktycz, M. J., Modrich, P. Warmack, R. J., and Allison, D. P. (2000) Identifying Sequence Similarities Between DNA Molecules. Ultramicroscopy. 82: 237-244.
Allison, D. P. Doktycz, M. J. Hoyt, P. R. Thundat, T. Warmack, R. J. (1997) The analysis of genomes by atomic force microscopy. Scanning 19(3) 136-137.
Hoyt, P. R., Bartholomew, C., Davis, A. J. Yutzey, K., Gamer, L. W., Potter, S. S., Ihle, J. N., and Mucenski, M. L. (1997) The Evi-1 proto-oncogene is required at midgestation for neural, heart, and paraxial mesenchyme development. Mechanisms of Development 65: 55-70.
Allison, D. P. Kerper, P. S., Doktycz, M. J., Thundat, T., Modrich, P., Larimer, F. W., Johnson, D. K., Hoyt, P. R., Mucenski, M. L., Warmack, R. J. (1997) Mapping individual cosmid DNAs by direct AFM imaging. Genomics 41: 379-384.
Lab Members (top)
