Michael Massiah

	Title	Associate Professor
	Office	149A NRC
	Phone	405-744-3797
	Email	michael.massiah@okstate.edu
	Degree	Ph.D. Chemistry & Biochemistry. 1996. University of Maryland Baltimore County, Catonsville, MD
	Website

Summer Undergraduate Research Program

Research Area (top)

Role of midline-1 in cellular differentiation

Midline-1 plays important roles in cell differentiation along the ventral midline in vertebrates during embryogenesis. Mutations of midline-1 result in heart/brain/kidney defects and craniofacial abnormalities. Midline-1 also targets a critical enzyme PP2A for degradation. PP2A regulates pathways in the cells are involved in metabolism, cell division, and cell death. In our lab, we are using NMR spectroscopy and other biophysical techniques to determine the structure and mechanism of action of midline-1.  Part of the mechanism of function we are deciphering, is how Midline-1 six distinct domains work together to target microtubule-associated protein phosphatase 2A (PP2A) for ubiquitin-mediated degradation. Results from our work will contribute to the understanding of key steps in embryogenesis, cancer, and the cellular processes that result in birth defects.

Selected Publications (top)

Tao, H, Simmons, B.N, Singireddy S., Jakkidi M., Short, K.M., Cox, T.C., Massiah, M.A., 2008. Structure of the MID1 tandem B-boxes reveals an interaction reminiscent of intermolecular RING heterodimers, Biochemistry 47, 2450-2457.

Tao, H, Simmons, B.N, Singireddy S., Jakkidi M., Short, K.M., Cox, T.C., Massiah, M.A. (2007) Structure of the MID1 tandem B-boxes reveals an interaction reminiscent of intermolecular RING heterodimers, Biochemistry, Web Release Date: 26-Jan-2008.

Massiah,M.A., Matts, J.A.B., Simmons, B.N. Short, K.M., Zou, Y., Cox, T.C. (2007) Solution Structure of MID1 B-box2 domain: Insights into an evolutionary conserved RING fold, Journal of Molecular Biology, 369, 1-10.

Massiah, M.A. Simmons, B.N, Short, K.M, Cox, T.C. (2006) Solution Structure of the RBCC/TRIM B-box 1 Domain of Human MID1; B-box with a Ring, Journal of Molecular Biology, 358, 532-545.

Simeonov, M, Altuve, A, Massiah, M.A., Wang, A., Eastman, M.A., Benson, D.R., and Rivera, M. (2005) Mitochondrial and Microsomal Ferric Cytochromes b5 Exhibit Divergent Conformational Plasticity in the Context of a Common Fold, Biochemistry, 44(26), 9308-19.

Mildvan, A.S., Xia, Z., Azurmendi, H.F., Saraswat, V., Legler, P.M., Massiah, M.A., Gabrelli, S.B., Bianchet, M.A., Kang, L.W., Amzel, L.M. (2005) Structures and mechanisms of Nudix hydrolases, Archives of Biochemistry and Biophysics, 433, 129-143.

Azurmendi HF, Wang SC, Massiah MA, Poelarends GJ, Whitman CP, Mildvan A.S. (2004) The roles of active-site residues in the catalytic mechanism of trans-3-chloroacrylic acid dehalogenase: a kinetic, NMR, and mutational analysis, Biochemistry. 43(14):4082-91.

Massiah, M.A., Saraswat, S., Azurmendi, H.F., Mildvan, A.S. (2004) Solution Structure, Mutagenesis, and NH Exchange Studies of the MutT-Mg²⁺-8-oxo-dGMP complex, 2003, J. Molecular Structure, 700, 247-254.

Massiah, M.A., Saraswat, S., Azurmendi, H.F., Mildvan, A.S. (2003) Solution Structure and NH Exchange Studies of the MutT Pyrophosphohydrolase Complexed with Mg²⁺ and 8-oxo-dGMP, a Tightly Bound Product, Biochemistry, 43, 10140-10154.

Saraswat, V., Massiah, M.A., Lopez, G., Amzel, L.M., Mildvan, A.S. (2002) Interactions of the products, 8-Oxo-dGMP, dGMP, and Pyrophosphate with the MutT Nucleoside Triphosphate Pyrophosphohydrolase, Biochemistry, Web Release 12-12-02.

Legler, P.M., Massiah, M.A., Mildvan, A.S. (2002) Mutational, Kinetics, and NMR Studies of the Mechanism of E. coli GDP-Mannose Mannosyl Hydrolase, an Unusual Nudix Enzyme, Biochemistry, 41 (35), 10834-10848.

Mildvan, A.S., Massiah, M.A., Harris, T.K., Marks, G.T., Harrison, D.H., Viragh, C., Reddy, P.M., Kovach, I.M. (2002) Short, Strong Hydrogen Bonds on Enzyme: NMR and Mechanistic Studies, Journal of Molecular Structure, 615, 163-175.

Marks, G.T., Harris, T.K., Massiah, M.A., Mildvan, A.S., Harrison, D.H.T. (2001) Mechanistic Implications of Methylglyoxal Synthase Complexed with Phosphoglycolohydroxamic Acid as Observed by X-ray crystallography and NMR spectroscopy, Biochemistry, 40 (23), 6805-6818.

Dinkova-Dostova, A.T., Massiah, M.A., Bozak, R.E., Hicks, R.J., Talalay, P. (2001) Potency of Michael reaction acceptors as inducers of enzymes that protect against carcinogenesis depends of their reactivity with sufhydryl groups, Proc. Natl. Acad. Sci., 98 (6), 3404-3409.

Czerwinski, R.M., Harris, T.K., Massiah, M.A.*, Mildvan, A.S., Whitman, C.P. (2001) The Structural Basis for the Perturbed pKa of the Catalytic Base in 4-Oxalocrotonate Tautomerase: Kinetic and Structural Effects of Mutations of Phe-50, Biochemistry, 40(7), 1984-1995.

Lab Members (top)