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Estela Arrese

	Title	Associate Research Professor
	Office	147 NRC
	Phone	405-744-7505
	Email	destela@okstate.edu
	Degree	Ph.D. Biochemistry. 1991. National University of La Plata, Argentina.
	Website

Research Area

Mobilization of triacylglycerol stores in insects

Triacylglycerol (TG) is the predominant form of storage of fatty acids and comprises the main energy reserve in all animals. The fat body is the principal site for storage of lipids in insects. Cells store intracellular TG in lipid droplets, which are dynamic organelles with essential functions in the maintenance of energy balance. The organism accesses these stores via the action of lipases in a process controlled by evolutionary conserved proteins that in insects are called Lipid storage droplets (Lsd). We have identified and characterized several proteins located at the center of the regulation of TG metabolism in insects. Of particular importance are the main fat body lipase (TGL), Lsd1 and Lsd2. Our studies are focused in the understanding of mechanism of function of these proteins.

Selected Publications

Howard AD, Verghese PB, Arrese EL, Soulages JL. 2011. The β-subunit of ATP synthase is involved in cellular uptake and resecretion of apoA-I but does not control apoA-I-induced lipid efflux in adipocytes.. Mol Cell Biochem. Feb; 348(1-2):155-64.

Howard AD, Verghese PB, Arrese EL, Soulages JL. 2010. Characterization of apoA-I dependent lipid efflux from adipocytes and role of ABCA1. Mol Cell Biochem. 343(1-2):115-24.

Jagadeeswaran G, Zheng Y, Sumathipala N, Jiang H, Arrese E, Soulages J, Zhang X and Sunkar R. 2010. Deep sequencing of small RNA libraries reveals dynamic regulation of conserved and novel microRNAs and microRNA-stars during silkworm development. BMC Genomics. 11:52

Arrese EL, Howard AD, Patel RT, Rimoldi OJ and Soulages JL. 2010. Mobilization of lipid stores in Manduca sexta: cDNA cloning and developmental expression of fat body triglyceride-lipase, TGL. Insect Biochemistry and Molecular Biology.40 (2):91-9.

Arrese E L and Soulages J L. 2010. Insect Fat Body: Energy, Metabolism, and Regulation. Annu. Rev. Entomol. 55:207-225.

Arrese EL, Mirza S, Rivera L, Howard AD, Chetty PS, and Soulages JL. 2008. Expression of lipid storage droplet 1 may define the role of AKH as lipid mobilizing hormone in Manduca sexta. Insect Biochemistry and Molecular Biology. 38: 993-1000

Verghese PL, Arrese EL, Howard AD, and Soulages JL. 2008. Brefeldin A inhibits cholesterol efflux without affecting the rate of cellular uptake and resecretion of apolipoprotein A-I in adipocytes. Arch Biochem Biophys. 478(2):161-166

Arrese EL,Rivera L, Hamada M, and Soulages JL. 2008. Purification and Characterization of Recombinant Lipid Storage Protein-2 from Drosophila melanogaster. Protein Pept Lett. 15:1027-1032

Arrese EL, Rivera L, Masakasu H, Mirza S, Hartson S, Weintraub S, and Soulages JL. 2008. Function and structure of lipid storage droplet protein 1 studied in lipoprotein complexes. Arch. Biochem. Biophys. 473, 42-47.

Arrese EL, Rivera L, Masakazu H, and Soulages JL. 2008. Purification and Characterization of Recombinant Lipid Storage Protein-2 from Drosophila melanogaster. Protein and Peptide Letters 15, 1027-1032.

Verghese PB, Arrese EL, Howard Smith AD, and Soulages JL. 2008. Brefeldin A Inhibits Cholesterol Efflux Without Affecting The Rate Of Cellular Uptake And Resecretion Of Apolipoprotein A-I in Adipocytes. Arch. Biochem. Biophys. 478, 161-166.

Arrese EL, Mirza S, Rivera L, Howard AD, Chetty PS, and Soulages JL. 2008. Expression of lipid storage droplet protein-1 may define the role of AKH as a lipid mobilizing hormone in Manduca sexta. Insect Biochem MolecBiol. 38, 993-100

Arrese, EL, Patel RT, and Soulages JL. 2006. The main triglyceride-lipase from the insect fat body is an active phospholipase A(1): identification and characterization. J Lipid Res. 47:2656-2667.

Patel RT, Soulages JL, and Arrese EL. 2006. Adipokinetic hormone-induced mobilization of fat body triglyceride stores in Manduca sexta: role of TG-lipase and lipid droplets. Arch Insect Biochem Physiol 23:73-81.

Patel RT, Soulages JL, Hariharasundaram B and Arrese EL. 2005. Activation of the lipid droplet controls the rate of lipolysis of triglycerides in the insect fat body. J Biol Chem. 280: 22624-22631.

Patel R, Soulages JL, Wells MA and Arrese EL. 2004. cAMP-dependent protein kinase of Manduca sexta phosphorylates but does not activate the fat body triglyceride lipase. Insect Biochem Mol Biol, 34(12):1269-1279.

Chetty PS, Arrese EL and Soulages JL. 2003. In vivo lipoprotein binding assay of the insect exchangeable apolipoprotein, apolipophorin-III. Protein Pept Lett, 10(5): 469-473.

Garda, H.A., Arrese, E.L., and Soulages, J.L. 2002. Structure of apolipophorin-III in discoidal lipoproteins: Interhelical distances in the lipid bound state and conformational change upon binding to lipid. J Biol Chem. 277:19773-19782.

Arrese, E.L., Canavoso, L.E., Jouni, Z.E., Pennington, J.E., Tsuchida, K., and Wells, M.A. 2001. Lipid storage and mobilization in insects: current status and future directions. Insect Biochem Mol Biol 31:7-17.

Arrese, E.L., Gazard, J.L., Flowers, M.T., Soulages, J.L., and Wells, M.A. 2001. Diacylglycerol transport in the insect fat body: evidence of involvement of lipid droplets and the cytosolic fraction. J Lipid Res 42:225-234.

Arrese, E.L., Flowers, M.T., Gazard, J.L., and Wells, M.A. 1999. Calcium and cAMP are second messengers in the adipokinetic hormone-induced lipolysis of triacylglycerols in Manduca sexta fat body. J Lipid Res40:556-564.

Arrese, E.L., and Wells, M.A. 1997. Adipokinetic hormone-induced lipolysis in the fat body of an insect, Manduca sexta: synthesis of sn-1,2-diacylglycerols. J Lipid Res 38:68-76.

Arrese, E.L., Rojas-Rivas, B.I., and Wells, M.A. 1996. Synthesis of sn-1,2-diacylglycerols by monoacylglycerol acyltransferase from Manduca sexta fat body. Arch Insect Biochem Physiol 31:325-335.